The project will establish, in conjunction with the East Java EPA, a new bioassay method for the evaluation of the toxicity of discharges to rivers in East Java and the safety of drinking water. This will be unique and move past the chemical analytical approaches that are common around the world. Rivers and the associated environs in East Java are currently highly polluted and discharge standards are based on concentration rather than volume and type of pollutants and the EPA is limited in terms of analytical capabilities. The safety of drinking water is also not guaranteed. The new methods will provide the ability to link discharges to the acute toxicity of greater than 3000 compounds as well as measure the coliform status of water, cheaply and effectively. The methods will allow a new and higher order regulatory framework to be established without the need for expensive analytics. The new framework is an essential building block in achieving SDG 6 for this region.